Anti-TRAF7 Antibody Picoband™

Product Name

Anti-TRAF7 Antibody Picoband™

View all TRAF7 Antibodies

SKU/Catalog Number

A05972-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TRAF7 Antibody Picoband™ catalog # A05972-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-TRAF7 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05972-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human TRAF7 recombinant protein (Position: R29-C670).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A05972-1 is reactive to TRAF7 in Human

Applications

A05972-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

75 kDa

Calculated molecular weight

74.609kDa

Background of TRAF7

Tumor necrosis factor (TNF; see MIM 191160) receptor-associated factors, such as TRAF7, are signal transducers for members of the TNF receptor superfamily (see MIM 191190). TRAFs are composed of an N-terminal cysteine/histidine-rich region containing zinc RING and/or zinc finger motifs; a coiled-coil (leucine zipper) motif; and a homologous region that defines the TRAF family, the TRAF domain, which is involved in self-association and receptor binding.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25 µg/ml, Human
Flow Cytometry, 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of TRAF7 using anti-TRAF7 antibody (A05972-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human T-47D whole cell lysates,
Lane 5: human A549 whole cell lysates,
Lane 6: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAF7 antigen affinity purified polyclonal antibody (Catalog # A05972-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAF7 at approximately 75 kDa. The expected band size for TRAF7 is at 75 kDa.

Click image to see more details

Figure 2. Flow Cytometry analysis of U87 cells using anti-TRAF7 antibody (A05972-1).
Overlay histogram showing U87 cells stained with A05972-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRAF7 Antibody (A05972-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For TRAF7 (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp586I021; E3 ubiquitin-protein ligase TRAF7; EC 6.3.2.-; MGC7807; RFWD1RING finger protein 119; RNF119RING finger and WD repeat-containing protein 1; TNF receptor-associated factor 7ring finger and WD repeat domain 1 TRAF7 CAFDADD, RFWD1, RNF119 TNF receptor associated factor 7 E3 ubiquitin-protein ligase TRAF7|RING finger and WD repeat-containing protein 1|RING finger protein 119|RING-type E3 ubiquitin transferase TRAF7|TNF receptor-associated factor 7, E3 ubiquitin protein ligase|ring finger and WD repeat domain 1

For more info on TRAF7, check out the TRAF7 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TRAF7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the TRAF7 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis